LSM 880 Airyscan NLO/FCS Confocal Microscope

Zeiss Axio Observer Z1 inverted microscope.

Airyscan 32-channel ultra-sensitive area detector for superresolution imaging (140 nm in x, y at 488 nm) at very low laser power suitable for live cell imaging with all common fluorescence labels.

Laser lines:
     Argon:  458, 488, 514 nm.   25 mW
     Diode: 405 nm.
     DPSS: 561 nm
     HeNe: 633 nm.
     Mai Tai DeepSee Tunable Laser:  690-1040 nm.

ZEN  2.1 software with the following modules:

      Z-stacks
     Experiment Designer
     Multi Channel
     Image Analysis
     Time Lapse
     Temperature and CO₂ controlled stage incubator for imaging live samples
     Definite Focus
     Point scanning photo stimulation (1P, 2P)
     Spectral unmixing   
     Colocalization
     Measurement
     FCS extended
     FLIM PicoQuant

HXP fluorescence illuminator and fluorescent filters for ocular viewing:

     Filter Set 43:  Alexa 546, Bodipy, Cy3, DsRed, dTomato, mOrange, Rhodamine
     Filter Set 49: DAPI, Hoechst, BFP, Indo-1.
     Filter Set 38: GFP, Alexa 488, CFP, FITC.

Objectives:  Fluorescence, Brightfield and DIC

     EC Plan-Neofluar 10x/0.30 NA  WD=5.2 mm
     Plan-Apochromat 20x/0.8 NA  WD=0.55 mm
     Plan-Apochromat 40x/1.3 NA  oil
     C-Apochromat 40x/1.2 NA  Water or Type W oil
     Plan-Apochromat 63x/1.40 NA oil
     Alpha Plan-Apochromat 100x/1.46 NA oil

Spectral detection using 32 channels PMTS (2) and ultra-sensitive GaAsP detector

BIG-2 GaAsP detector as NDD for 2P and FLIM detection