The Yale Flow Cytometry Facility on Science Hill provides comprehensive services to support flow cytometric analysis and cell sorting (also known as FACS). These services include access to advanced instrumentation, expert technical assistance, user training, and consultation.
Book FACS Machines
PPMSFacility Manager
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Freya Wencker, Ph.D.
Lecturer in Molecular, Cellular, and Developmental Biology
What is Flow Cytometry?
Flow cytometry is a powerful technique that enables simultaneous measurement and analysis of multiple physical and chemical characteristics of individual particles—typically cells—as they pass through a series of laser beams in a fluid stream at rates of thousands of particles per second. Properties assessed include cell size, internal complexity (granularity), and fluorescence intensity (across up to ~12 fluorescence channels for analysis of different proteins or fluorophores), which are detected using an optical-electronic system that captures how cells scatter light and emit fluorescence signals.
Particles or cells ranging from 0.5 to 150 micrometers in size can be analyzed, and single-cell suspensions of animal or plant tissues can be analyzed. In addition to analysis, the facility offers cell sorting (FACS) to isolate specific populations or individual cells for downstream applications including microscopy, biochemical assays, functional analyses, or cell line generation.
FACS Aria III sorter
The FACS Aria III sorter has 5 lasers, UV 355nm, V 405nm, B 488nm, and R 635nm. It can detect 18 colors.
Conditions of Usage
All samples must be filtered through 35-40um mesh filters immediately before sorting to help prevent blockages. Researchers will be charged for the time taken to clear blockages, and subject to subsequent bookings, the availability of your sorting time may be reduced.
Minimum booking is 1 hour after which users will be charged in 30-minute increments. Users will be charged based on the time booked or time used, whichever is longer. The manager reserves the right to alter online bookings post-sort to reflect the time used (if longer than that booked).
The Facility manager reserves the right to change booking times dependent on the machine’s availability, but will give researchers as much notice as possible.
Fees
Assisted Cytometry: $130 per hour
Self-sorting: $85 per hour
Analysis: $48 per hour
Hours
Monday: 9am-5pm
Tuesday: 9am-7pm
Wednesday: 9am-5pm
Thursday: 9am-5pm
Friday: 9am-4pm
After hours booking is also available for sorting without a technician.
BD FACS LSR Fortessa X20
The Fortessa has 4 lasers, V 405nm, B 488nm, and R 635nm. It can detect 16 colors.
Conditions of Use
All samples must be filtered through 35-40um mesh filters immediately before sorting to help prevent blockages. Researchers will be charged for the time taken to clear blockages, and subject to subsequent bookings, the availability of your sorting time may be reduced.
Minimum booking is 1 hour after which users will be charged in 30-minute increments. Users will be charged based on the time booked or time used, whichever is longer. The manager reserves the right to alter online bookings post-sort to reflect the time used (if longer than that booked).
The Facility manager reserves the right to change booking times dependent on the machine’s availability, but will give researchers as much notice as possible.
Fees
Assisted Cytometry: $130 per hour
Analysis: $48 per hour
Hours
Monday: 9 AM - 5 PM
Tuesday: 9 AM - 7 PM
Wednesday: 9 AM - 5 PM
Thursday: 9 AM - 5 PM
Friday: 9 AM - 4PM